Thus the demand for marker free transgenic plants is one of the most popular tendency. Several techniques to eliminate marker genes have been developed.
因此，获得无选择标记的转基因植株就成为植物基因工程发展的一大趋势，现已发展出了几种技术来实现这一目标。
参考来源 - 双TIn this study,the ovary-drip method with higher transformation frequency and reproducibility was established for soybean. In addition,a marker-free and vector-free AlNHX1 gene cassette was transferred into soybean via ovary-drip method.
本研究优化了一种转化率较高、重复性较好的大豆子房滴注法,通过该方法将无载体骨架序列无选择标记的AINHX1基因元件导入大豆,提高了大豆的耐盐性,为其它功能基因在大豆转化中的应用奠定了基础。
参考来源 - 大豆子房滴注转化方法的研究及耐盐基因（AlNHX1）的转化In this study,the ovary-drip method with higher transformation frequency and reproducibility was established for soybean. In addition,a marker-free and vector-free AlNHX1 gene cassette was transferred into soybean via ovary-drip method.
本研究优化了一种转化率较高、重复性较好的大豆子房滴注法,通过该方法将无载体骨架序列无选择标记的AINHX1基因元件导入大豆,提高了大豆的耐盐性,为其它功能基因在大豆转化中的应用奠定了基础。
参考来源 - 大豆子房滴注转化方法的研究及耐盐基因（AlNHX1）的转化·2,447,543篇论文数据，部分数据来源于NoteExpress
为了使马铃薯抗晚疫病基因RB能够在番茄育种和生产中应用，构建了RB无选择标记载体。
We construct a marker-free vector of RB gene in order to apply with RB in the tomato late blight breeding and production.
具有快速响应、灵敏度高、选择性强、无标记分子等特点。
The quasi-one-dimensional metal oxide nano-material biosensor has the characteristics of rapid response, high sensitivity, strong selectivity, no labeled molecule and the like.
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