The gene XYL1 and its expression element was cloned from previously established yeast expression plasmid pACT2-xyl1 and then fused with yeast chromosome integration vector YIp5-KanR, resulting in recombinant plasmid YIp5-KanR-x1.
将木糖还原酶基因XYL1连同其表达元件从前期已构建好的酵母表达质粒pACT2-xyl1中克隆下来并将其与酵母染色体整合载体YIp5-KanR进行连接,构建出重组染色体整合质粒YIp5-KanR-x1。
参考来源 - 代谢木糖产乙醇的酿酒酵母重组菌株的初步构建·2,447,543篇论文数据,部分数据来源于NoteExpress
肌注外源质粒后,外源质粒在小鼠细胞内逐渐被清除,外源质粒不会整合到宿主染色体上。
Foreign plasmid DNA was cleared from cellular system in short time after intramuscular injection. Foreign plasmid can not integrate into host chromosome genomic in the immunized mice.
方法基于基因融合分析在转录研究中的实用性,构建了两种PQS相关对照质粒和整合突变株。
Methods Based on the usefulness of gene fusion analysis for transcriptional analysis, two kinds of plasmids and integrant mutant strains were developed for the systemic study of PQS.
How would I put a gene that I'm interested in into a plasmid?
怎么把我想要的基因整合到质粒上呢
How did we put our gene fragments into this plasmid DNA in order to make multiple copies of it, or to clone the gene?
如何把基因片段整合到质粒DNA上,来获得大量拷贝,或者说来克隆基因呢
Let's assume that we have this plasmid cloning vector and we have some pieces of DNA that we would like to put into a plasmid that we would like to make copies of.
假设我们已经有了克隆质粒载体,也有了我们想要将之,整合到质粒上的某些DNA片段,同时也是我们所要复制的DNA片段
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