方法采用方阵滴定法，比较4种封闭液、不同稀释度的捕获抗体、检测抗体和辣根过氧化酶标记链菌素对检测结果的影响，确定ELISA法的最佳实验条件。
Method 4 kinds of block buffer, the different concentration of anti-MIF and biotin-anti-MIF, the different dilution of streptavidin-HRP are used for titration of ELISA test.
对产生信号的标记抗体-抗原复合物的捕获，是用抗体固定在已经提到的硝酸纤维素的特定区域内来达到的。
The capture of the labeled antibody-analyte complex for signal generation can be achieved with the antibody immobilized in a defined area of nitrocellulose membrane as mentioned.
对复性后的单链抗体以抗原捕获ELISA法进行检测。
The refolded single-chain antibodies were examined by antigen capture ELISA.
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