红外光谱指纹区(1300~400cm-1,7.69~ 25微米)吸收峰的特征性强,可用于区别不同化合物结构上的微小差异。犹如人的指纹,故称为指纹区。指纹区的红外吸收光谱很复杂,能反映分子结构的细微变化。这个区域的振动类型复杂而且重叠,特征性差,但对分子结构的变化高度敏感,只要分子结构上有微小的变化,都会引起这部分光谱的明显改变。
通过对甘薯蔓多糖红外光谱指纹区的特征吸收解析表明:甘薯蔓多糖含有羧基,是一种中偏酸性多糖;
According to the analysis of IR fingerprint characteristics absorption, the result showed that the PSPV was an acidic polysaccharide containing a carboxyl.
方法采用PCR指纹法、基因内间隔区(IGS)和磷脂酶基因(PLB1)测序分析鉴定基因型。
Methods Genotype was characterized by PCR fingerprinting, intergenic spacer (IGS) sequencing, and phospholipase (PLB1) gene sequencing.
目前,DNA指纹技术已在玉米品种区试审定、品种权测试、品种权执法等方面发挥了重要的作用,具有良好的应用前景。
At present, DNA fingerprinting has played an important role in regional trials, variety testing and enforcement, which has a bright future for application.
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