方法随机抽样确定安塞县0~39岁人群269名，采集血清标本，检测脊灰抗体。
Methods We collected randomly 269 serum samples among 0-39 years healthy population and detected the polio neutralizing antibody in Ansai County.
方法采用多阶段抽样方法，随机抽取638名调查对象进行个案调查并采集血液标本分离血清，用血凝抑制（HI）方法对标本进行检测。
Methods Using multi-level sampling method, 638 subjects were selected randomly and surveyed. The serological specimens from 638 subjects were separated and detected using haemoglutinin inhibition(HI).
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