【Key words】 Carrot; Antifreeze protein gene; Clone; Sequence; Vector construction; Agrobacterium; Genetic transformation; Tissue culture; Tomato;
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异源超表达抗冻蛋白基因能赋予敏感宿主植物抗冻能力。
The heterologous over-expression of genes encoding AFPs in freezing-sensitive plants can enhance the freezing resistance of host plants.
目的构建可用于大肠杆菌表达系统的含抗冻蛋白基因afp的表达载体。
Objective: to obtain the procaryotic expression vector containing the antifreeze protein gene AFP.
目前,应用于植物抗寒基因工程的基因包括两大类:植物抗寒相关基因及鱼的抗冻蛋白基因。
There are two kinds of genes being used in plant cold-resistant genetic engineering:plant cold-resistant relative genes and fish antifreeze protein genes.
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