以B5培养基为基础培养基，添加不同浓度的蔗糖、琼脂及不同激素组合进行试验，对油菜DH系的组培技术进行优化。
B5 as basic medium appended with different concentrations of sucrose, AGAR and different hormone combinations was used to optimize the culture technique for DH line in Brassica napus l.
现代细菌学研究的方法学始于19世纪晚期，包括建立细菌染色方法和在培养基中进行微生物分离的技术，当时的培养基是用明胶和琼脂固化的营养物质。
The modern methods of bacteriological technique began in the late 19th century with the use of stains and the development of methods of cultivating organisms on plates of nutrients.
采用血清琼脂糖凝胶区带电泳技术分析未经处理和处理后的蛋白尿样本。
The serum agarose gel zone electrophoresis technique was adopted to analyze the non-treated and treated samples of proteinuria.
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