方法从人胚胎海马区分离神经干细胞，采用无血清培养基，进行体外扩增培养、传代。
Methods The serum free culturing technology was used to isolate, culture and pass neural stem cells from embryonic human hippocampus.
方法：取健康新西兰幼兔骨髓，密度梯度离心法分离骨髓间充质干细胞并进行体外扩增培养、成骨诱导分化及鉴定。
METHODS: Bone marrow was obtained from healthy New Zealand rabbits, by using density gradient centrifugation BMSCs were isolated and were then cultured in vitro.
方法：利用无血清培养和细胞克隆培养技术，从胚胎大鼠海马、纹状体、脊髓等区分离神经干细胞，进行体外扩增培养、传代、贴壁分化观察。
Methods: The advantage of serum free and clone culturing technology was performed to isolate, culture and passage neural stem cells from embryonic rat hippocampus, striatum and spinal cord.
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