前言：目的：探讨高效的原代成年大鼠下颌骨成骨细胞培养方法，为进一步的实验研究奠定基础。
Objective: To investigate an efficient method of primary mandibular osteoblasts culture, and establish a foundation for further experimental study.
结论酶交替消化法原代培养胎鼠成骨细胞，获得的成骨细胞纯度高、数量大，可作为一种相对可靠、有效的原代成骨细胞培养方法。
Conclusion Osteoblasts achieved by this method are pure with large quantities, so this method can be used as a reliable and efficient way of primary culture.
方法应用体外细胞培养技术观察MC3T3 - E1成骨细胞株在不同处理的牙骨质表面的生长状况。
Methods Cell culture technique in vitro had been used to study the growth condition of MC3T3-E1 osteoblast line on the surface of cementum treated with various methods.
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