是指直径在60-250μm,能适用于贴壁细胞生长的微珠。一般是由天然葡聚糖或者各种合成的聚合物组成。
用同体积生物反应罐,基本培养条件相同,采用批培养、再循环培养、灌流培养三种方式进行了Vero细胞微载体(Cytodex I)的周期培养。三种培养方法均达到预期效果,最终细胞密度分别为每毫升2.09×106、3.37×106、4.34×106。
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Objective To develop a method to prepare human articular cartilage derived microcarrier for both rapid propagating chondrocytes and being used as scaffold to support chondrogenesis.
目的制备一种既能大量扩增人软骨细胞,又能作为细胞支架的人关节软骨源性微载体。
参考来源 - 人关节软骨源性微载体的制备The microcarrier technique of cell cultivation offers an attractive method for cell amplification and enhancement of phenotype expression, which has been proven to be more effective than that of cell culture on flat substrates such as culture dishes.
微载体技术则为这种细胞的培养提供了一个有效的方法。 大量研究发现,与传统的平板培养法相比较,微载体技术更适合大量细胞的培养,该技术能有效的扩增细胞并增强细胞的显性表达。
参考来源 - 作为细胞微载体的明胶缓释微球的制备及其性能研究·2,447,543篇论文数据,部分数据来源于NoteExpress
方法:建立微载体-基质细胞体外造血模型。
Methods: The hematopoietic model for stromal cell adhering to microcarrier was established.
本发明公开了一种动物细胞培养用微载体及交联方法。
The invention discloses a microcarrier for culturing animal cell and a crosslinking method thereof.
目的探讨由微载体培养的L 0 2人肝细胞和中空纤维舱构成的生物反应器的生物效能。
ObjectiveTo investigate the biological function of bioreactor consisting of cultivated human liver cell line L-02 on microcarriers and hollow fiber tube.
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