目的研究DNA循环测序中一些常见因素的影响。
Objective to study the influence of some factors on DNA cycle sequencing.
方法：PCR循环测序法是将PCR扩增与核酸序列分析相结合的一种研究方法。
Methods: PCRbased cycle DNA sequencing is a method that combines PCR amplification with DNA sequencing.
采用大引物方法，利用质粒多克隆位点两侧的普通测序引物作为旁侧引物，在单个PCR管内，经2个步骤共34个循环进行定点突变。
Using normal sequencing primers adjacent to multiple clone sites of plasmid as flanking primers, a novel megaprimer method was performed in one tube with two steps and 34 cycles.
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