Besides, Primers were designed to do amplification and sequencing analysis for the patients’and normals’COL1A1 exon in the famaily, then compare the results of sequencing the corresponding gene with the GeneBank sequence comparison of normal to search the mutation.
设计引物对此耳硬化症家系的COL1A1基因外显子进行扩增和测序分析,测序结果与GeneBank中相应基因的正常序列对比寻找突变位点。
参考来源 - 一耳硬化家系的COL1A1基因突变分析Alignment of DNA and cDNA sequences showed that there introns existed in DNA sequence whose length was 52bp,69bp and 64bp, respectively.2.
序列对比后发现绿色木霉内切几丁质酶基因含有三个内含子,大小分别为52bp、69bp、64bp。
参考来源 - 绿色木霉内切几丁质酶基因的克隆及其在大肠杆菌中表达的研究·2,447,543篇论文数据，部分数据来源于NoteExpress
研究人员对比了非洲象和亚洲象，以及来自猛犸象和美洲乳齿象的细胞核dna序列。
The researchers compared sequences of DNA from the nuclei of African and Asian elephants, and from woolly mammoths and the American mastodon.
通过对比找出人类基因中相同序列，就可以推测和相应的生物模型中的基因有类似的功能。
Computer comparison then shows which human genes correspond in DNA sequence and thus, presumably, in function, to the genes in these "model" organisms.
通过与公开的1500种细菌的参照序列进行对比，他们排除了人类DNA，并尽可能多地鉴定出其中的细菌种类。
By comparing the DNA sequences with publicly available reference sequences for 1500 bacterial and other species, they excluded all human DNA and identified as many bacteria as they could.
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