结论利用同源序列克隆进化上保守的基因是简捷快速的方法。
Conclusion It is a simple and rapid method to clone gene through homologene sequence.
构建SARS冠状病毒S基因序列克隆p - SARS - S，作为自行设计、建立的RT - p CR检测方法的阳性对照，并为该基因的表达奠定基础。
To construct a clone p-SARS-S containing SARS coronavirus s gene fragment as the positive control of RT-PCR detect methods, which can build the basis for the expression of the gene.
这两个克隆都包含有一段DNA序列，能在在细胞周期蛋白d1的RNA被转录的时候将荧光蛋白绑定到其上。
Both clones included a DNA sequence that allow a fluorescent protein expressed in the cell to bind to cyclin D1 RNA the moment it is transcribed.
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