指示性病例是一位40岁女子，她于2011年1月17日出现症状，2011年2月1日经阿比让巴斯德研究所采用免疫酶联试验（ELISA）检测IgM抗体呈阳性反应。
The index case was a 40 year old female, who developed symptoms on 17 January 2011 and tested positive for IgM by ELISA test conducted by Institut Pasteur in Abidjan on 1 February 2011.
目的：研究不同分泌信号对豹蛙酶（ONC）在巴斯德毕赤酵母中分泌效率的影响。
Objective:To investigate the effects of different secretion signals on efficiency of onconase(ONC) secretion in Pichia pastoris expression system.
为了获得大量有活性的重组蛋白酶便于今后的工业应用，我们将蛋白酶WF146克隆到分泌性表达宿主枯草芽胞杆菌和巴斯德毕赤酵母中。
For the convenience of production and purification, Bacillus subtilis and Pichia pastor is were used as hosts for the expression of protease WF146.
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