目的探讨用转粒细胞巨噬细胞集落刺激因子(GM CSF)基因瘤苗,联合白介素12 (IL 12)治疗小鼠t细胞淋巴瘤的方法。
Objective To study the method of immunotherapy against t cell lymphoma by tumor cells transfected by GM-CSF gene combined with interleukin 12 (IL-12).
方法联合应用粒细胞—巨噬细胞集落刺激因子(GMCSF)、白细胞介素4(IL4)诱导培养小鼠脾脏单个核细胞分化为DC,并从形态学、表型及功能方面对其加以检测。
Methods Mononuclear cells isolated from mice spleen were induced into DC by GMCSF and IL4, and then detected with their morphology, phenotype and function.
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