将文库菌落印迹至尼龙膜，分区培养提取质粒DNA ，建立基因池，并分别转染NIH/ 3T3细胞。
The plasmids in cDNA library and in gene pools were extracted and NIH/3T3 cells were transfected respectively.
用毛细转移法将凝胶上的DNA转移至正电荷尼龙膜上。
The DNAs in the agarose gel then were transfered to positively charged nylon membrane by capillary transfer.
应用推荐