这个实验采用转篮法，对五个时间点的样品进行吸光度测定，并且设计了空白对照和标准对照。
Methods: we adopted the rolling basket method to measure absorption degree of 5 samples, blank control and standard control.
然后分别提取84例拟进行造血干细胞移植病人及家系成员的外周血d NA，采用RSCA和序列特异性引物体外基因扩增(PCR ssp)法平行对照对HLA A基因位点进行分型。
DNA samples of related hematopoietic stem cell transplant donor-recipients were extracted from peripheral blood cells. HLA-A loci were typed both by RSCA and PCR-SSP.
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