目的：介绍一种简便、有效的定点突变技术。
Objective: To develop a simple and efficient way to perform site-directed mutagenesis.
目的：利用聚合酶链反应定点突变技术构建人血小板反应素1基因第13外显子编码钙结合域突变体。
AIM: To establish the mutant of coding calcium binding fragment of the 13th exon of human thrombospondin-1 (TSP-1) gene with polymerase chain reaction (PCR) site directed mutagenesis technology.
着重阐述了基因定点突变技术、基因融合技术和翻译修饰技术等新兴定点固定化技术的原理、特点和操作。
The principles, operation and applications of site-directed mutagenesis, gene fusion technology, and post-translational modification methods were introduced emphatically.
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