目的构建木瓜凝乳蛋白酶的表达载体，并在大肠杆菌中表达。
Objective To construct recombinant plasmid for expression of chymopapain in E.
研究发现，重组的碱性蛋白酶在进入大肠杆菌周质空间时可能存在前肽自动脱落的现象。
The study also discovered that while the recombinant alkaline protease was secreted into the periplasmic space of e.
用脱脂奶琼脂平板法检测了1 0 0株大肠杆菌的胞外蛋白酶。
The extracellular proteases(ECPase) of 100 Escherichia coli strains were detected by using the skim milk plate.
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