目的：通过使用3末端碱基游移混合引物，减少引物末端错配，提高多变区基因片段的PCR检测阳性率。

Objective: To reduce the risk of 3 -terminal mismatch between primers and template and increase the sensitivity of polymerase chain reaction (PCR) in the detection of variable region of DNA.

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雨水在许多地方可能会变得更加密集与多变，处在干旱和半干旱热带区的农民可能会变得更加脆弱。

Rain was likely to become more intense and variable in many places, and farmers in the arid and semi-arid tropics were likely to become more vulnerable.

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伯德认为，这一地区多变的天气状况和不断上升的气温可能有利于这种热带鸟儿的生存。

Changing weather patterns and warmer temperatures in the region have likely helped the tropical birds survive, bird said..

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