是恢复原有性质的意思。变性的生物大分子恢复成具有生物活性的天然构象的现象。变性的一种逆转。
...:生物大分子的天然构象遭到破坏导致其生物活性丧失的现象。蛋白质在受到光照,热,有机溶济以及一些变性济的作用时,次级键受到破坏,导致天然构象的破坏,使蛋白质的生物活性丧失。复性(renaturation):在一定的条件下,变性的生物大分子恢复成具有生物活性的天然构象的现象。
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大肠埃希菌表达的含PTD穿膜序列的融合 蛋白3种复性方法比较 - 淘金者论文 关键词】 蛋白转导域 复性 包涵体 [gap=1089]Key words] protein transduction domain; refolding; inclusion bodies(IBS)
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重复性 [科技] repeatability ; RSD ; repeability ; Reproducibility
恢复性增长 recovery growth
报复性关税 [税收] retaliatory duty ; [税收] retaliatory tariff ; [税收] retaliatory customs duty
重复性关键词 Repeated Words ; Repeated d Words ; RepeatedWords
可重复性 [计] repeatability ; reproducibility ; repeatability reproducibility ; Repeatable
重复性误差 [测] repeatability error ; reproducibility error ; Cv ; repeatability error of a measuring instrument
报复性 Retaliatory ; Vindictive Damages ; vengefulness
反复性动作损伤 repetitive motion injury
IB solubilized in the presence of 8mol/L urea were diluted with renaturation solutions so that folding process initiated. Concentrated IL-3 were digested with thrombin and authentic rhIL-3 released.
超声破碎细菌细胞得包涵体,经洗涤处理可使包涵体纯度达90%,用8mol/L尿素变性溶解包涵体沉淀后直接稀释复性,再超滤浓缩、凝血酶消化,释放天然型rhIL-3。
参考来源 - 大肠杆菌表达的人重组ILPurification of the irrational, Commonly, the recombinant arginine deiminase proteins are inactive and inclusion bodies in cells, and it is very critical to renature. The reported purification methods are generally diluted renature followed by purification.
纯化方法的不合理,主要是因为重组精氨酸脱亚胺酶一般是以无蛋白活性的包涵体形式表达,蛋白复性这一步就比较关键,报道过的纯化方法一般是先稀释复性然后再纯化,而稀释复性后的蛋白浓度很低,在浓缩蛋白时以及后面的纯化过程中由于精氨酸脱亚胺酶的不稳定性,很容易引起酶蛋白的活性损失。
参考来源 - 重组精氨酸脱亚胺酶的表达、纯化、复性及其生物活性研究These results provided further evidence that HPHIC is an effective tool in the refolding and purification of recombinant proteins.
结果进一步证明HPHIC法是同时复性和纯化重组蛋白的有效工具。
参考来源 - 采用不同疏水相互作用色谱柱从包涵体中快速制备重组人干细胞因子(英文)The activity of expressed protein after renaturation had recovered and further confirmed by lymphocyte proliferation assay in vitro.
ELISA鉴定复性后的蛋白已恢复活性,并能在体外刺激外周血单个核细胞的增殖。
参考来源 - 人源鼻咽癌双价抗独特型抗体疫苗的制备及活性鉴定·2,447,543篇论文数据,部分数据来源于NoteExpress
拘捕增加了对报复性攻击的恐惧。
有人在谈论实施报复性封锁,以阻止供应物资通过。
There's been talk of a retaliatory blockade to prevent supplies from getting through.
这些妇女因恶劣的工作条件而患上了折磨人的重复性劳损。
The women developed painful RSI because of poor working conditions.
If I then put it back into its original condition, lower the heat say, temperature back to body temperature and reduce the pH down to seven again, the molecules will re-nature.
假如我把烧杯的温度恢复到初始状态,降低温度,温度降低到体温,将pH值降低到七,这些分子会复性
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