A Bt-E. coli shuttle vector pHT315 was deleted its replication region of Bt ,then constructed a novel vector named pHT315-1 which composed a multiple cloning site,erythromycin and ampicillin-resistance marker and could only replicated in E. coli.
Bt-E.coli穿梭载体pHT315去除Bt复制区，构成了一个具有红霉素和氨苄抗性，同时具有多克隆位点的只能在E.coli中复制的载体，命名为pHT315-1。
参考来源 - 苏云金芽孢杆菌cry基因克隆表达和质粒复制区克隆的研究·2,447,543篇论文数据，部分数据来源于NoteExpress
这可以在文件数据被复制到内部缓存区时立即释放用于保留该数据的NFS缓存页面。
This frees up NFS cache pages used to hold file data as soon as they have been copied to internal buffers.
例如，可以从后端系统检索信息，但不将每个可能的对象均从存储区复制到内存中，而仅加载其中的小部分，将其放置在缓存中。
For example, you can retrieve information from a backend system, but rather than copy every possible object from the store to memory, just load small pieces and put them into a cache.
复制工作区中的原始模块，并将它们重构为新名称，以反映它们是原始版本。
Copy the original modules in the workspace and refactor them to new names to reflect that they are the original version.
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