本文通过电镜观察研究了抗冻剂的种类、浓度，以及降温速率和复温方法等因素对狗气管低温保存效果的影响；
The effects of cooling and thawing velocity on trachea's cryopreservation with different concentration of cryoprotect agents were studied.
通过对香葱的预处理、速冻、冻干脱水及复水性研究，确定了相应的预处理工艺参数及冻干工艺参数。
The pretreatment, quick-frozen, freeze-dry dehydration and rehydration of shallot are analyzed. The corresponding pretreatment technology, eutectic point and freeze dry curve are decided.
人卵冻贮：在成功玻璃化冷冻小鼠卵母细胞的基础上，再随机选用人卵母细胞，其冷冻方法、复温方法及统计学处理同动物实验。
Human experiment: Human oocytes were collected from our center, the cryopreserved method, rewarming method and statistical analysis method is same to animal experiment.
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