结果酶切和测序证实PTEN基因克隆和真核表达载体构建成功。

Results Restriction enzyme analysis and DNA sequence analysis showed that PTEN gene was cloned and the eukaryotic expression vector was constructed successfully.

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目的对丙型肝炎病毒(HCV) NS5B区基因克隆并测序，研究其变异状况。

Objective to clone and sequence HCV NS5B gene, and to investigate the mutation of the gene.

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自1985年克隆出了第一个冰核基因后，目前已经从冰核细菌中克隆了8个冰蛋白基因并完成了测序。

First active gene had been cloned by 1985. After that, the 8 ice genes had been gotten and sequenced in the past years.

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