目的利用同源性分析，克隆定位新的人类间隙连接蛋白基因，并探讨该基因和遗传性耳聋的关系。
Objective To clone a novel human connexin gene and find out the relationship between this gene and hereditary deafness.
所克隆的奶山羊blg基因调控区与山羊blg伪基因显著不同，其同源性仅为83 %(上游调控区)和88%(下游调控区)。
The dairy goat gene differed significantly from goat BLG pseudo gene with a homology of only 83% (upstream region) and 88% (downstream region), respectively.
结果挑选了4个差异筛选的阳性克隆进行测序，序列同源性分析表明它们均与胰岛素- i基因序列高度一致。
Results four positive clones of differential screening were picked out for sequencing. Homology analysis indicated that all of the four clone sequences were the same as that of insulin-I gene.
应用推荐