方法将森林脑炎病毒感染地鼠肾单层细胞，收获病毒液，经超滤浓缩、柱层析纯化病毒后配制而成。
Methods Hamster kidney cells were infected with tick - borne encephalitis virus, and the harvested virus suspension was concentrated by ultrafiltration and purified by column chromatography.
用其体外转染地鼠肾细胞BHK-21，经间接免疫荧光法（IFA）检测，证实了JEV-E的瞬时表达。
The transient expression of JEV E in BHK 21 cells was detected by indirect immunofluorescence assay(IFA) with a monoclonal antibody 2H4 against JEV E.
探讨在原代地鼠肾细胞组织培养的狂犬病疫苗(PHKCV)工业化生产中，对病毒滴度与疫苗效力进行测定，并对数据进行分析。
During the process of industrialized production of primary hamster kidney cell vaccine (PHKCV), We determined the virulence and potency and analyzed the data.
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