受体菌 recipient, recipient bacteria,在细菌的遗传物质转移中,把接受遗传物质的细菌称为受体菌。
来自受体菌自身的同源质粒,因克服了宿主的限制一修饰性,可以极显著地提高电转化效率。
A significant increase of transformation efficiency was revcaled with the homologous plasmid isolated from recipient itself since the effects of host restriction and modification werc avoided.
选取NG4、NG31、NG43、NG70进行细菌的接合试验,以观察耐药质粒能否通过接合进行传递以及传递后受体菌耐药性的变化。
NG4, NG31, NG43 and NG70 were selected to perform the experiment of conjugation, from this we can determine whether the resistant plasmids could be transferred through conjugation.
目的:克隆并表达艰难梭菌毒素A羧基末端受体结合区基因。
AIM: to obtain the high expression of the gene coding for clostridium difficile toxin a receptor binding zone.
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