反向转录是以mRNA为模板,以4SRNA或色氨酸(t)RNA为引物,4种dNTP为原料,根据碱基配对原则。遗传信息流动方向是从RNA到DNA,正好与中心法则遗传信息流向和转录过程相反的过程。
端粒酶,是一种催化延长端粒末端的反向转录酶。
Telomerase is a kind of ERT which causes catalysis and prolong the end of telomere.
采用定量反向转录-聚合酶链式方法,观察急性重复性低氧对小鼠脑内葡萄糖转运蛋白1,3基因表达的影响。
Quantitive RT PCR was used to observe the effect of repeated acute hypoxic exposure on the expression of glucose transporter protein 1 and 3 genes and tolerance in the mice brain.
双轮回‘聚合酶链式反应’(PCR)和‘反向转录酶-聚合酶链式反应’(RT - PCR)被用来检测基因CYP1A1的转录水平。
A double - round reverse transcriptase - coupled polymerase chain reaction (RT - PCR) was applied to detect the CYP1A1 transcript.
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