同时合成相应的正义链与随机链 ,其序列分别为 :反义链 (AODN) :5′ GGAGCGCGCGGCATC 3;随机链 (RODN ) :5′ AGCTGGCACCGGCGG 3′。
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另一条链(互补链或反义链)的序列总是与第一条链相匹配,即C与G,A与T,反过来也一样。
The other (complementary or antisense) strand always has a sequence that matches the first strand, with each C complemented by a G, and each A by a T, and vice versa.
So you made somehow an anti-sense polynucleotide to the insulin gene or some fraction of the insulin gene.
这样你就合成了,胰岛素基因的反义多聚核苷酸链,或者说胰岛素基因的片段
The one that does is called the sense strand and the other one is called the anti-sense strand, anti meaning it's the compliment and it will hybridize to the sense.
能够进行编码的一条链叫做正义链,另一条则叫做反义链,反义的意思是它与正义链互补,并能够与之杂交
If somehow you could take your anti-sense molecules that you've made and you could get them into cells, then by this process of hybridization they would naturally form a pair like this.
如果你能得到合成的反义链,并能使它进入细胞,那么通过杂交,它们就能自然形成这样的一对
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