哺乳动物细胞对双链RNA不识别意味着可用短发卡RNA来阻断信使RNA,从而使相应基因的信号无法传递。

This aversity to double-stranded RNA means short hairpins can be used to knock out the messengers, thus nullifying the signal from the underlying gene.

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重组酶与核酸酶不同，它剪断基因的双链，然后在断点的周围等候。

Unlike nucleases, recombinases cut a double-stranded piece of DNA and then wait around on the exposed ends.

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Cerepro被固定在一个腺病毒(一种能够携带双链形式的DNA病毒)上来传染基因，这个基因负责产生胸苷激酶这种蛋白质。

Cerepro harnesses an adenovirus, a type of virus which carries its DNA in double-stranded form, to transmit a gene responsible for the production of a protein called thymidine kinase.

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When it's double stranded this gene can't be translated, because you have to have the single strand in order for the transfer RNA to bind and for this process of translation to take place.

当基因处于双链结构状态时,是不能进行翻译的,因为必须把双链拆开得到单链,才能结合转运RNA并开始翻译过程

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The first step is to take the DNA fragments that we're interested in and put them into this vector by basically cutting open the double stranded DNA and inserting the gene that we like in the region where we've cut.

第一步是把我们感兴趣的DNA片段,整合到这个载体里,基本的方法是切开质粒这个双链DNA,把我们想要的基因插入切开的区域

耶鲁公开课 - 生物医学工程探索课程节选