胰腺组织单细胞悬液制备方法的比较研究_临床医学论文--《医药卫生护理学》--互联网考试中心 关键词: 单细胞悬液; 胰 腺; 流式细胞术 [gap=784]key words: single cell suspension; pancreas; flow cytometry
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骨骼肌在制成单细胞悬液过程中的人为损伤,可造成DNA提前降解。
The artificial damage of the skeletal muscle during the preparation of single cell suspension could cause the earlier degradation of the DNA.
方法:采用流式细胞术对30例新鲜肺癌组织和5例正常对照组组织制成的单细胞悬液进行了DNA含量分析。
Methods:We analyzed the single-cell suspensions of 30 fresh lung carcinoma tissues and 5 normal tissues of control group to get their DNA levels by using flow cytometry.
结论消化振荡法对人胚胎神经干细胞的活性影响较小,是一种有效、安全的将神经球打散成单细胞悬液的实验方法。
Conclusion This study suggests a valid and safe method that can be used for efficient passage of neural stem cells.
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