方法利用无血清悬浮培养、单细胞克隆技术分离神经上皮干细胞；
Methods Serum free cells suspension culture and single cell cloning technique were used to isolate neuroepithelial stem cells.
来自细胞悬浮培养物的条件培养基能显著地促进人参培养细胞的单细胞在较低细胞植板密度培养时的克隆形成。
Conditioned medium (CM) stemming from cell suspension culture could remarkably promote the formation of single cell clone from culture cells of Panax ginseng below a critical cell plating density.
经3～ 4次继代培养即可得到悬浮的单细胞。
Suspension single cells were obtained after successive subculturing of 3 or 4 generations.
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