结果1．单管巢式PCR反应条件中的模板量及外引物循环数对检测结果的影响较大。
Result (1)It is affected to the PCR results by the quantities of templates and outer cycles in one tube nested PCR.
背景：移植后造血干细胞的顺利归巢是单倍相合骨髓移植成功的关键。
BACKGROUND: Successful homing of haemopoietic stem cells is the key step for the success of haploidentical bone marrow transplantation.
目的探讨单管巢式聚合酶链反应(SNPCR)检测石蜡包埋组织结核分支杆菌dna的特异性和敏感性。
Objective To study the sensitivity and specificity of single tube nested polymerase chain reaction (SN PCR) technique in detecting Mycobacterium tuberculosis DNA in paraffin embedded tissues.
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