经过验证,对其中活性变化明显的7个启动子用邻硝基苯基-β半乳糖苷(ONPG)作为底物进行表达活性测定.结果表明,获得的强启动子比原来的提高了3~8倍,而弱启动子则活性下降明显,其中3个几乎无活性.
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fragilis)为出发菌株,甲基磺酸乙酯(EMS)为诱变剂,5-溴-4-氯-3-吲哚-吡喃 半乳糖苷 (X-gal)作为筛子从诱变后的860个菌落中选育到一株具有较高转苷活力的β-D- 半乳糖苷 酶高产菌株,菌株编号为脆壁克鲁维酵母(K.
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...半乳糖苷酶催化,转变为半乳糖。后者作为一种诱导剂分子结合阻遏蛋白,使蛋白构象变化,导致阻遏蛋白与O序列解离、发生转录。异丙基硫半乳糖苷(IPTG)是一种作用极强的诱导剂,不被细菌代谢而十分稳定,因此被实验室广泛应用。 IPTG的优点 1.
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半乳糖苷酶 [生化] galactosidase ; LACZ ; GAL ; Tilactase
异丙基硫代半乳糖苷 IPTG ; isopropyl thiogalactoside
鞘氨醇半乳糖苷 [生化] psychosine
硫代半乳糖苷 IPTG ; isopropylthiogalactoside
呋喃半乳糖苷 galactofuranoside
α-半乳糖苷酶 Galactosidase ; alpha-galactosidase ; Galactosidase preparation from green coffee beans ; EC
呋喃半乳糖苷酶 galactofuranosidase
脑半乳糖苷脂 cerebrogalactoside
半乳糖苷酶试验 ONPG
The enzyme targeted specifically to α-galactoside linkages.
酶专一性水解α-D-半乳糖苷键。
参考来源 - 短双歧杆菌αIt is a kind of nonreductive oligosaccharides. Its constitutional formula is fructose-glucose-galactose-galactose with molecular formula C_(24)H_(42)O_(21), and molecular weight is 666.59.
水苏糖是从一种叫做地灵(Stachys floridona)的根茎中提取的α半乳糖苷类非还原性低聚糖,简单的结构表示为:果糖—葡萄糖—半乳糖—半乳糖(Galα1,6-Galα1,6-Gluβ1,2-Fructose),分子式为C_(24)H_(42)O_(21),分子量为666.59,无毒,易溶于水。
参考来源 - 猪到人异种心脏移植超急性排斥反应的研究·2,447,543篇论文数据,部分数据来源于NoteExpress
就半乳糖苷酶的应用研究,尤其是半乳糖苷酶遗传修饰酵母在酒精发酵中的作用进行了简要综述。
The applications of these enzymes, especially the functions of genetically modified yeasts in ethanol production are reviewed briefly.
目的制备白藜芦醇脂质体(RES - lip)和半乳糖苷修饰的白藜芦醇脂质体(RES - GLIP),探讨并比较二者的肝靶向作用。
Aim to prepare resveratrol liposome (RES-LIP) and the liposomes modified by a galactoside (RES-GLIP) as well as to investigate and compare their liver targeting effect.
在这项工作中,我们更详细研究的定位 个别核筹措的诱导 半乳糖苷酶,公益少年团的推动者NPTII在压制和 引起国家和估计实际核 转移。
In this work, we studied in more detail the positions of individual nucleosomes on the inducibleGAL–CYC promoter of NPTII in the repressed andinduced states and estimated the actual nucleosomeshift.
The promoter in this case is called the lac promoter and normally in micro-organisms that lac promoter is used to produce a gene called lacZ which makes a protein called beta-galactosidase.
在这个例子中的启动子称作lac启动子,通常在微生物中,lac启动子是用来生成,一段叫lacZ的基因,这段基因,能制造一种蛋白叫β-半乳糖苷酶
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