结论:共刺激分子CD 154和ICOS的全血基因表达量能可靠的区分排异患者和对照患者。
CONCLUSIONS: The whole blood gene expression quantities of costimulatory molecules CD154 and ICOS reasonably robustly differentiated rejection patients from control patients.
方法:新鲜抗凝全血,经CD 14单克隆抗体标记,采用流式细胞仪区分单核细胞及其表面CD 14的表达。
Methods: Fresh anticoagulant blood was labelled by CD14 monoclonal antibody, then monocytes were identified and the expressions of CD14 were tested by flow cytometry.
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