而这么做我要切割更多的组织。
那位指挥切割的老渔民告诉我,还是在三年前,他们也曾捕到这样大的鲨鱼,不得不用斧子砍断鱼骨,把鱼骨、鱼头砍下来。
The village elder who directed the cutting said that the last time they caught a shark as big as this was three years ago, and they had to use an axe to cut through the bone and separate the head.
我们还没讲过转动,但你们可以对于常温可以有些直观感觉,如果我这样子挥手,我切割到的分子,会转的更快。
But you probably have just an intuitive feeling that at ordinary temperatures, if I do this, if I wave my hand in the air, molecules that I happen to intersect are going to start spinning faster.
If I cut up just DNA from the chromosome, what's called genomic DNA, then I'm going to have both exons and introns within the fragments that I create.
如果我切割的恰好是染色体上的,基因组DNA,那我切成的片段上,就会同时有外显子和内含子
If I cut both the plasmid and my DNA of interest with the same restriction enzyme I'm going to end up with the same sticky ends on both molecules.
如果用同一种限制性内切酶,来切割质粒和我感兴趣的DNA,在两个分子上就能得到同样的粘性末端
If I took this same restriction enzyme and tried to cut the chromosomal DNA of a sickle patient, it wouldn't cut at that point because the wrong sequence is there.
而如果我用同样的限制酶,去切割病人的染色体DNA,那该酶是不会起作用的,因为基因序列不对
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