利用磁吸附细胞分选法(MACS)分离大鼠脾脏CD 4 + T细胞,流式细胞术检测MACS分选前后CD 4 + T细胞比例,监测细胞分选效果。
Magnetic absorption cell sorting (MACS) method was used to isolate the CD4 + t lymphocytes from the spleen of the rats. Flow cytometry was used to monitor the efficiency of isolation.
无端的高梯度介质带在分选空间循环通过,从而实现了非磁性物与磁性物的不断分离。
The endless high gradient matrix belt circulates through the separating zone, thus providing continuous separation of magnetic minerals from non-magnetic minerals.
通过多年的实践与理论分析,获得的最佳分选效果,分选后的杂质可小于1%,即分选效果最高可达99.9%,并且属于完全物理分离,分离后各种材料的性质都不改变,不影响再生利用。
Through years of practice and theoretical analysis, we obtain the best separation result and the impurity after separation is smaller than 1% and the best result can reach 99.9%.
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