必须选择适合免疫共沉淀抗体识别目的蛋白表位的细胞裂解条件。
The choice of lysis conditions must be tailored to the nature of the epitope recognized by the immunoprecipitating antibody.
方法：以TRF1抗体应用免疫共沉淀方法，从细胞蛋白抽提物中分离TRF1蛋白复合物，并作蛋白质肽指纹谱鉴定；
Methods: The co immunoprecipitation assay was employed to isolate TRF1 protein complex and the immunoprecipitate was subjected to MALDI TOF mass spectrometry for protein identification.
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