There are three parts in this thesis. Two new and simple strategies were established for fabricating of deoxyribonucleic acid(DNA) electrochemical biosensor based on covalent immobilization of probe NH2–ssDNA by sol–gel coupling (the first chapter) and diazotization-coupling (the second chapter).
本论文分三部分,第一、第二部分利用溶胶-凝胶自组装法和偶氮化法共价固定NH2–ssDNA,研制了两种新型的DNA传感器;第三部分是在第二部分的基础上,在电极表面共价固定硫堇分子,制备了一种无酶无试剂型过氧化氢传感器。
参考来源 - 基于共价固定法构筑DNA生物传感器的研究·2,447,543篇论文数据，部分数据来源于NoteExpress
文章叙述了利用辐射接枝技术，缩合共价法，使葡萄糖氧化酶固定在聚乙烯、氟46膜上。
The paper is described the immobilization of GOD on polyethylene and F_ (46) by radiation grafting and condensation covalent.
该结果说明，酶的“柔性固定化”模型可以改善传统共价结合固定化酶及手臂固定化酶活力回收率不高的缺陷。
The flexible immobilized model could achieve a higher recovery of immobilized enzyme activity than traditional covalent immobilization and arm spacer immobilization.
固定化方法主要有吸附法、包埋法、共价键合法和交联法。
Immobilization technology included absorption, entrapment, covalent binding and cross-linking methods.
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