为研究IBV变异株的基因组及其变异特征，本实验通过基因扩增拼接的方法获得了肾病变型毒株JH06111的全基因组序列。
To study the genomic characteristics of IBV variant isolate, we obtained the full genome sequences of nephropathogenic JH06111 by amplifying, cloning and sequencing of its cDNA.
方法从确诊的HIV - 1感染者全血样本中提取基因组dna，经套式聚合酶链反应(PCR)扩增和测序。
Methods Genomic DNA was extracted from the whole blood samples collected from HIV-1 positive patient. Nested PCR was carried out and PCR products were purified and used directly for sequencing.
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