方法设计出针对各片段的特异性引物,用P CR方法从Z 37病毒感染的细胞提取细胞总rna,逆转录扩增、产物克隆t载体,纯化后测序,测定的序列应用DNASTAR软件比较分析。
Methods the total RNA was extracted from Z37 virus infected cells and the RT-PCR products were cloned into t vector, sequenced and analyzed by DNASTAR software.
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