方法将人肝门部胆管癌标本进行细胞培养，传代。
Methods The fresh human hilar cholangiocarcinoma specimen was cultured.
方法用狗肾传代MDCK细胞培养法观察儿茶提取物抑制甲型流感病毒的致细胞病变作用；
Methods The inhibitory cytopathic effects (CPE) of catechu extract on influenza A virus were observed by MDCK cell culture techniques.
方法：采用细胞培养的方法建立稳定传代的胰腺癌hs 766t细胞系，体外培养人脐带静脉内皮细胞。
Methods: We built the pancreatic cancer cell group HS 766t and human endothelium cell in the umbilical vein by the way of cell culture in vitro.
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