目的：探讨腔外单滑结技术在无钛夹二孔法腹腔镜胆囊切除术（LC）中的效果。
Objective:To explore the effects of out-cavity single-slip-knot technique in non-titanium clip two-hole method laparoscopic cholecystectomy(LC).
吹十孔口琴一般都是用第一种，吹和弦或者使用舌堵法时就要用第二种口形了。
Use the first mouth shape to play single notes and the second one to play chord with tongue block.
葡萄籽经有机溶剂梯度法及酶法提取，大孔树脂初步纯化，薄层色谱法分离纯化得到原花青素单体和二聚体。
The monomer and dimer of proanthocyanidins in grape seeds were obtained by gradient and enzymatic extraction, isolation with macroporous adsorption resin, and purification by TLC.
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