以10%的脱脂乳为冻于悬浮基质，对蔗糖、血清蛋白、甘油、海藻酸钠四种保护剂的保护效果进行了研究。
Using 10% nonfat dried-milk as suspended substrate, the function of four protectors including sucrose, serum protein, glycerin and trehalose were studied.
程序降温法：以甘油和二甲基亚砜为低温保护剂，分别以不同的比例添加到含有细胞悬液的冻存管中。
Programmed cooling method: The glycerol and Dimethylsulfoxide, as cryoprotective agents, were mixed with COC1 cells suspensions at different concentration, respectively.
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