方法采用大鼠胚胎中脑神经元细胞进行体外培养。
Methods Using dissociated cell culture of rat embryo in vitro.
研究神经生长因子（NGF）对中脑神经元的突起生长和促生存作用。
Objective The effects of nerve growth factor ( NGF ) on the survival and neurite growth of mesencephalic neuron were obsetved.
用表达ntn的条件培养基处理原代培养胚胎中脑神经元，发现其有促进神经元存活和发育功能。
It was found that NTN can promote survival and growth of neurons when culturing primary embryonic mesencephalic neurons with conditioned medium contained NTN.
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