目的探讨彩超引导下脐静脉穿刺术的安全性和成功率。
Objective To evaluate the efficacy and safety of color-ultrasound guided percutaneous umbilical blood sampling.
方法采用流式细胞仪技术对不同培养时间和不同培养条件下脐血cd 34 +细胞中pcna的表达水平和细胞周期进行了测定。
Methods Using flow cytometry, PCNA and cell cycle in CB CD34 + cells were measured in different conditions and different culture time.
方法在相同细胞因子组合下，用体外液体培养比较脐血有核细胞程序扩增组与一次扩增组及有无基质层条件下的优劣。
Methods under the same condition with hematopoietic factors in the liquid culture, compared the sequential expansion group and once for all expansion group with or without stroma cell layer.
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