一步生长实验 one step-growth experi-ment 一步生长实验 one step-growth experi-ment 用感染菌群调查噬菌体的生活史或增殖的方法。在此实验中,为了避兔由所生的后代噬菌体的再吸附造成的麻烦,要高度稀释感染细胞的悬浮液,每隔一定时间采取一部分,在直接地或人为地使之溶菌后,测定感染中心。如果在一定条件下使用一定的噬菌体和细菌,则与重复度无甚关系。可得到一定的暗期潜伏期、释放数的值。
根据一步生长实验结果绘制出了一步生长曲线。
One-step growth curve was determined according to the results of one-step growth experiments.
方法采用宿主范围测定、噬菌斑计数、负染色电镜观察、血清中和试验及血清交叉中和试验、一步生长实验等技术观察分离的噬菌体。
Methods the phage was identified by detection of the host range, calculation of phage plaque, negative staining for TEM, serological neutralization test and one step growth experiment.
结论细胞生长过程上的各项主要指标控制稳定,细胞在本系统中生长状态良好,为下一步的实验奠定了坚实的基础。
Conclusion Every important index in the process of growing on cells keep stable and cells is in a good state in this system, which establish a stable basis for further experiments.
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