Amplification VP7 gene from RV G4 serotype, construct a plasmid includes this gene and clone culture.
从轮状病毒G4型中扩增出vp7基因，将该基因构建于质粒并克隆培养。
In this study, VP7 gene and CTB-VP7 fusion gene expression vectors were constructed, and a high-efficient genetic transfomation system of carrot(Daucus carota L. )
本实验以轮状病毒的VP7为目的基因，构建了VP7基因的表达载体和VP7-CTB融合表达载体；
PCR detection results showed that 30 plants showed positive in 37 kanamycin resistance plants. Its indicated that the foreign gene VP7 had already integrated into genome of receptor plants.
P CR检测结果表明，37株卡那霉素抗性植株中30株为阳性，说明vp7外源基因已整合到受体植物基因组中。
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