Methods: the two-electrode voltage clamp recording technique was used to observe the ligand-gated and voltage-gated ion channel currents expressed in Xenopus laevis oocytes without follicles.
方法：在去除滤泡膜的非洲爪蟾卵母细胞上，建立双电极电压钳记录技术，并使用该技术观察爪蟾卵母细胞表达的内源性递质门控性和电压门控性离子通道电流。
Our results indicate that voltage-gated ion channel currents recorded by Clampex will be more accurate and reproducible by properly using P/N leak subtraction.
结果表明， 在采集电压门控性离子通道电流时， 合理地利用P/N漏减会使离子电流记录更为准确可靠。
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