The selection of the microwave parameter: The 2ml vitrification solution which was cryoperserved by vitrificaion was direct thawed with microwave.
第二部分，筛选出与微波参数匹配的适合于胚脑细胞的玻璃化溶液。
参考来源 - 微波复温在胚脑细胞玻璃化冻存过程中的作用研究The optimized concentrations for each component in the vitrification solution were 52%(w/w) EG,8%(w/w) glucose and 3%(w/w) CS.
由此获得的玻璃化溶液的组成是:载体溶液-乙二醇-葡萄糖-硫酸软骨素,其中,乙二醇、葡萄糖和硫酸软骨素的质量百分比浓度分别为52%、8%和3%。
参考来源 - 牛角膜内皮细胞玻璃化冷冻保护剂及组织低温断裂消除方法的研究·2,447,543篇论文数据，部分数据来源于NoteExpress
Objective: to study the suitable vitrification solution and to find a efficient protocol to preserve newborn rat ovaries.
目的：探索适宜新生鼠卵巢保存的玻璃化液和冷冻方案。
Vitrification method consists of 5 steps mainly: loading, dehydration by protective solution of vitrification, cooling, warming and washing (namely unloading).
玻璃化法主要由装载、玻璃化保护液脱水、降温、复温、洗涤这5个环节构成。
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